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TNEB Bill Calculator

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TNEB Bill Calculator - 2021 (Revised on May 23, 2016) For Domestic Usage Only Units Consumed: Units Tariff Charges Unit Range Cost Fixed Subsidy From To ≤ 100 ₹ 0 ₹ 150 1 100 ₹ 1.50 ≤ 200 ₹ 20 ₹ 150 1 200 ₹ 1.50 ≤ 500 ₹ 30 ₹ 150 1 100 ₹ 1.50 101 200 ₹ 2.00 201 500 ₹ 3.00

RNA-RNA Interaction Prediction and Visualization

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Ribonucleic acid (RNA) is a linear polymeric molecule composed of four bases namely adenine (A), cytosine (C), guanine (G), and uracil (U). It is used for the prediction of various functions which include coding, decoding, regulation, and gene expression. The prediction of RNA–RNA interaction plays a major role in the study of structure folding and free energy of RNA complex molecule. This is a video tutorial for RNA-RNA interaction prediction using RactIP (RNA-RNA interACTion prediction using IP) and visualize inter and/or intramolecular base-pair interactions using VARNA (Visualization Applet for RNA) tools. The list of tools used is RactIP and VARNA .

Best Fonts to Display Biological Sequence and Alignment

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Biological sequence alignment is a method to find similarities between two sequences. The characters (letters or biological sequence alphabets) are arranged row-wise and column-wise according to match/identity and mismatch of characters through symbol representation. In row-wise, a gap character (hyphen symbol ‘-’) is used to align/adjust the sequence characters for matching without changing the order. Correspondingly, the column-wise characters such as match (same character, pipe symbol ‘|’, colon symbol ‘:’, asterisk symbol ‘*’, or dot symbol ‘.’), mismatch (anyone of the sequence character, or blank space ‘ ’), positive (positive symbol ‘+’), and gap/indel (blank space ‘ ’) are used to represent sequence alignment. A sequence or sequence alignment must be properly formatted in a text editor/word processor using fixed-width fonts to find the differences of characters in the sequence and control word wrapping. Formatting sequence helps to find the sequence length and formatting s

Creating Custom Database using Standalone NCBI BLAST+

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Basic Local Alignment Search Tool (BLAST) is a collection of programs developed using heuristic algorithm in C++ for comparing DNA, RNA, and protein sequences. The standalone command-line interface (CLI) of BLAST is named as BLAST+. The latest version of NCBI BLAST+ can be downloaded from the FTP server of NCBI ( ftp://ftp.ncbi.nih.gov/blast/executables/blast+/LATEST ). This is a simple tutorial for creating a custom database, accessing the database, and performing a sequence search using BLAST+. 1. Creating a Custom Database A nucleotide ( nucl ) or protein ( prot ) database can be created using -dbtype parameter in makeblastdb program. We can create two types of database using command-line below, Non-indexed Database: ./makeblastdb -in DBX.fasta -out DBX -dbtype prot Building a new DB, current time: 12/04/2020 10:10:06 New DB name: C:\NCBI\blast-2.6.0+\bin\DBX New DB title: DBX.fasta Sequence type: Protein Keep MBits: T Maximum file size: 1000000000B Adding sequences

Synthesis and Retrosynthesis of Molecule(s) for Drug Development using IBM RXN

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Synthesis is a step-by-step process of constructing complex molecules using a set of molecules and specific reagents. While retrosynthesis (retro- backwards) is the process of deconstructing a molecule into readily available simple molecules. IBM RXN is the first, FREE artificial intelligence (AI) web service for predicting reactions and retrosynthesis molecules. Moreover, the AI model is independent of atom-mapping and give back a confidence level of the prediction. In this tutorial, I have explained synthesis and retrosynthesis of a molecule using IBM RXN. List of resources used in this video tutorial are IBM RXN , and NCBI PubChem .

2D Plot and 3D Visualization of Protein-Ligand and Protein-Protein Interactions

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Intermolecular interactions are weak bonds formed due to electron sharing between two or more atoms to hold a molecule together. In molecular modelling or computer-aided drug designing (CADD), intermolecular interaction studies are performed to analyze the stability/energy of docking molecules. This video tutorial demonstrates two-dimensional (2D) plot and three-dimensional (3D) molecular visualization of protein-ligand and protein-protein inter-molecular interactions using LigPlot + /LigPlus tool. LigPlot + is a successor of original LigPlot program. The protein-ligand inter-molecular interactions are computed using LigPlot program and protein-protein or domain-domain inter-molecular interactions using DimPlot program. Moreover, the interactive 3D molecular visualization of the computed result can be viewed using RasMol or PyMOL. List of softwares used in this tutorial are LigPlot + , LigPlus , RasMol , PyMOL , and JDK .

Splitting PDB File into Chains and Ligands Without using Tools

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Protein Data Bank (PDB) file format is a representation of a three-dimensional (3D) structure of the protein and ligand data extracted by interpreting the experimental result of high-energy electromagnetic radiation (X-ray) or Nuclear Magnetic Resonance (NMR) through the computational method. The standard PDB file format was created by RCSB during the 1970s for parsing through software. The atomic coordinate entries of protein are represented by ATOM & TER fields, and ligand by HETATM field (given in the table below). Record Type Columns Description ATOM 1 - 4 "ATOM" 7 - 11 Atom serial number 13 - 16 Atom name 17 Alternate location indicator 18 - 20 Residue name 22 Chain identifier 23 - 26 Residue sequence number 27 Code for insertions of residues 31 - 38 X orthogonal Å coordinate 39 - 46 Y orthogonal Å coordinate 47 - 54 Z orthogonal Å coordinate 55 - 60 Occupancy 61 - 66 Temperature factor 73 - 76 Segment identifier 77 - 78 Element symbol 79 -